KDM5B | HUABIO HA750922 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

KDM5B

catalog :

HA750922

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-97

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of KDM5B on different lysates with Rabbit anti-KDM5B antibody (HA750922) at 1/2,000 dilution. Lane 1: BT-20 cell lysate Lane 2: MDA-MB-468 cell lysate Lane 3: AGS cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 176 kDa Observed band size: 145 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750922) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

image 2 :

KDM5B was immunoprecipitated in 0.2mg A549 cell lysate with HA750922 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750922 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: ER1802-21 IP in A549 cell lysate Lane 3: Rabbit IgG instead of ER1802-21 in A549 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds

product information

SKU :

HA750922

Target name :

KDM5B

Species reactivity :

Human

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human KDM5B aa 960-1,009 / 1,544.

Uniprot id :

Q9UGL1>SwissProt: Q9UGL1 Human

Host :

Rabbit

Clone number :

PSH03-97

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

BT-20 cell lysate, MDA-MB-468 cell lysate, AGS cell lysate, A549 cell lysate.

Molecular wt :

Predicted band size: 176 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage