Histone H3 (acetyl K4) | HUABIO HA750905 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H3 (acetyl K4)

catalog :

HA750905

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-71

reactivity :

human, mouse, rat

application :

western blot, chromatin immunoprecipitation, dot blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H3 (acetyl K4) on different lysates with Rabbit anti-Histone H3 (acetyl K4) antibody (HA750905) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 1 minute 22 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750905) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling Histone H3 (acetyl K4) with Rabbit anti-Histone H3 (acetyl K4) antibody (HA750905) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (acetyl K4) antibody (HA750905) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of C6 cells treated with or without 500ng/mL TSA for 6 hours labeling Histone H3 (acetyl K4) with Rabbit anti-Histone H3 (acetyl K4) antibody (HA750905) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 (acetyl K4) antibody (HA750905) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750905

Target name :

Histone H3 (acetyl K4)

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,ChIP,Dot Blot

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Histone H3 (acetyl K4) aa 1-50.

Uniprot id :

P68431>SwissProt: P68431 human;SwissProt: P84243 human;SwissProt: Q16695 human;SwissProt: Q6NXT2 human;SwissProt: Q71DI3 human;SwissProt: P68433 mouse;SwissProt: P84228 mouse;SwissProt: Q6LED0 rat

Host :

Rabbit

Clone number :

PSH03-71

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 500ng/mL TSA for 4 hours cell lysate, NIH/3T3 treated with 400nM TSA for 18 hours cell lysate, HeLa cells treated with 500ng/mL TSA for 4 hours, C6 cells treated with 500ng/mL TSA for 6 hours.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:500-1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin. ;Dot Blot: 1:1,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750905_4.jpg

Pic legend4 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated 500ng/mL TSA for 4 hours with Histone H3 (acetyl K4) (HA750905) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750905_5.jpg

Pic legend5 :

Dot blot analysis of Histone H3 (acetyl K4) on different proteins with Rabbit anti-Histone H3 (acetyl K4) antibody (HA750905) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys4) (negative) Lane 3: Di-Methyl-Histone H3 (Lys4) (negative) Lane 4: Tri-Methyl-Histone H3 (Lys4) (negative) Lane 5: Acetyl-Histone H3 (Lys4) (positive) Lane 6: Acetyl-Histone H3 (Lys9) (negative) Lane 7: Acetyl-Histone H3 (Lys27) (negative) Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 50 seconds.

more info or order :

HUABIO product webpage