SP110 | HUABIO HA750900 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SP110

catalog :

HA750900

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-66

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SP110 on different lysates with Rabbit anti-SP110 antibody (HA750900) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HeLa cell lysate Lane 3: HL-60 cell lysate Lane 4: SW480 cell lysate Lane 5: Daudi cell lysate Lane 6: K-562 cell lysate Lane 7: PC-3M cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 78 kDa Observed band size: 29-78 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750900) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SP110 antibody (HA750900) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750900) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750900

Target name :

SP110

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human SP110 aa 301-550 / 689.

Uniprot id :

Q9HB58>SwissProt: Q9HB58 Human

Host :

Rabbit

Clone number :

PSH03-66

Isotype :

IgG1

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, HeLa cell lysate, HL-60 cell lysate, SW480 cell lysate, Daudi cell lysate, K-562 cell lysate, PC-3M cell lysate, human lymph node tissue.

Molecular wt :

Predicted band size: 78 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:2,000

more info or order :

HUABIO product webpage