p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 | HUABIO HA750893 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13

catalog :

HA750893

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-59

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 on different lysates with Rabbit anti-p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 antibody (HA750893) at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: K-562 cell lysate (30 µg/Lane) Lane 5: MDA-MB-231 cell lysate (30 µg/Lane) Lane 6: COS-1 cell lysate (30 µg/Lane) Lane 7: RAW264.7 cell lysate (30 µg/Lane) Lane 8: Neuro-2a cell lysate (30 µg/Lane) Lane 9: PC-12 cell lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750893) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 with Rabbit anti-p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13 antibody (HA750893) at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane Lane 2: Human p38 alpha recombinant protein, 50ng/Lane Lane 3: Human p38 delta recombinant protein, 50ng/Lane Lane 4: Human p38 beta recombinant protein, 50ng/Lane Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750893) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750893

Target name :

p38 beta/MAPK11 + p38 gamma/MAPK12 + p38 delta/MAPK13

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MAPK12 aa 1-367 / 367.

Uniprot id :

Q15759>SwissProt: Q15759 Human;SwissProt: P53778 Human;SwissProt: O15264 Human;SwissProt: Q9WUI1 Mouse;SwissProt: O08911 Mouse;SwissProt: Q9Z1B7 Mouse;SwissProt: D4A3U7 Rat;SwissProt: Q63538 Rat;SwissProt: Q9WTY9 Rat

Host :

Rabbit

Clone number :

PSH03-59

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T cell lysate, HeLa cell lysate, MCF7 cell lysate, K-562 cell lysate, MDA-MB-231 cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cytoplasm, Nucleus, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000

more info or order :

HUABIO product webpage