MDA5 | HUABIO HA750890 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MDA5

catalog :

HA750890

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-56

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (HA750890) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Raji cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: HeLa cell lysate (20 µg/Lane) Lane 8: SH-SY5Y cell lysate (20 µg/Lane) Lane 9: A549 cell lysate (20 µg/Lane) Lane 10: A431 cell lysate (20 µg/Lane) Lane 11: Mouse pancreas tissue lysate (40 µg/Lane) Lane 12: Rat kidney tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 117 kDa Observed band size: 140/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750890) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750890

Target name :

MDA5

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MDA5 aa 250-1,025 / 1,025.

Uniprot id :

Q9BYX4>SwissProt: Q9BYX4 Human;SwissProt: Q8R5F7 Mouse;Entrez Gene: 499801 Rat

Host :

Rabbit

Clone number :

PSH03-56

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, HepG2 cell lysate, Jurkat cell lysate, Daudi cell lysate, Raji cell lysate, MCF7 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, A549 cell lysate, A431 cell lysate, mouse pancreas tissue lysate, rat kidney tissue lysate.

Molecular wt :

Predicted band size: 117 kDa

Subcellular location :

Cytoplasm, Nucleus, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000

more info or order :

HUABIO product webpage