Phospho-IRE1 (S724) | HUABIO HA750869 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-IRE1 (S724)

catalog :

HA750869

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH03-35

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-IRE1 (S724) on different lysates with Rabbit anti-Phospho-IRE1 (S724) antibody (HA750869) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 5 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750869) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-IRE1 (S724) on different lysates with Rabbit anti-Phospho-IRE1 (S724) antibody (HA750869) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: C2C12 starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750869) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of Jurkat cells treated with 100nM Calyculin A for 30 minutes labeling Phospho-IRE1 (S724) with Rabbit anti-Phospho-IRE1 (S724) antibody (HA750869) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-IRE1 (S724) antibody (HA750869) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750869

Target name :

Phospho-IRE1 (S724)

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Ser724 of human IRE1.

Uniprot id :

O75460>SwissProt: O75460 Human;SwissProt: Q9EQY0 Mouse

Host :

Rabbit

Clone number :

PSH03-35

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate, Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, C2C12 starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate, Jurkat cells treated with 100nM Calyculin A for 30 minutes, human pancreas tissue, HeLa cells treated with 100nM Calyculin A for 30 minutes.

Molecular wt :

Predicted band size: 110 kDa

Subcellular location :

Endoplasmic reticulum membrane; nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:500 ;IHC-P: 1:500 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750869_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue untreated / treated with λpp with Rabbit anti-Phospho-IRE1 (S724) antibody (HA750869) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750869) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750869_5.jpg

Pic legend5 :

Flow cytometric analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-IRE1 (S724). Cells were fixed and permeabilized. Then stained with the primary antibody (HA750869, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage