product summary
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company name :
HUABIO
product type :
antibody
product name :
Calsequestrin 2
catalog :
HA750857
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH03-16
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Calsequestrin 2 on different lysates with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/10,000 dilution and competitor's antibody at 1/5,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate (15 µg/Lane)
Lane 2: Mouse heart tissue lysate (15 µg/Lane)
Lane 3: Mouse brain tissue lysate (negative) (15 µg/Lane)
Lane 4: Rat skeletal muscle tissue lysate (15 µg/Lane)
Lane 5: Rat heart tissue lysate (15 µg/Lane)
Lane 6: Rat brain tissue lysate (negative) (15 µg/Lane)
Predicted band size: 46 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750857) at 1/10,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunofluorescence analysis of paraffin-embedded human skeletal muscle tissue labeling Calsequestrin 2 with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750857, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
image 3 :
Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle tissue labeling Calsequestrin 2 with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750857, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
product information
SKU :
HA750857
Target name :
Calsequestrin 2
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human Calsequestrin 2 aa 1-399 / 399.
Uniprot id :
O14958>SwissProt: O14958 Human;SwissProt: O09161 Mouse;SwissProt: P51868 Rat
Host :
Rabbit
Clone number :
PSH03-16
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse skeletal muscle tissue lysate, mouse heart tissue lysate, rat skeletal muscle tissue lysate, rat heart tissue lysate, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse heart tissue.
Molecular wt :
Predicted band size: 46 kDa
Subcellular location :
Sarcoplasmic reticulum lumen.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:10,000
;IHC-P: 1:1,000
;IF-Tissue: 1:500
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750857_4.jpg
Pic legend4 :
Immunofluorescence analysis of paraffin-embedded rat skeletal muscle tissue labeling Calsequestrin 2 with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750857, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA750857_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750857) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA750857_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750857) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA750857_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750857) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA750857_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750857) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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