RHOG | HUABIO HA750827 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RHOG

catalog :

HA750827

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-70

reactivity :

green monkey, human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RHOG on different lysates with Rabbit anti-RHOG antibody (HA750827) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Hela cell lysate (20 µg/Lane) Lane 5: MCF7 cell lysate (20 µg/Lane) Lane 6: PANC-1 cell lysate (20 µg/Lane) Lane 7: COS-1 cell lysate (20 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750827) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of HeLa cells labeling RHOG. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750827, 1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA750827

Target name :

RHOG

Species reactivity :

Human,Monkey

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human RHOG aa 1-50 / 191.

Uniprot id :

P84095>SwissProt:P84095 Human;SwissProt:A0A0D9QW62 Green monkey

Host :

Rabbit

Clone number :

PSH02-70

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, MDA-MB-231 cell lysate, K-562 cell lysate, Hela cell lysate, MCF7 cell lysate, PANC-1 cell lysate, COS-1 cell lysate, HeLa.

Molecular wt :

Predicted band size: 21 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;FC: 1:1,000

more info or order :

HUABIO product webpage