MAN2A1 | HUABIO HA750824 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MAN2A1

catalog :

HA750824

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-67

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MAN2A1 on different lysates with Rabbit anti-MAN2A1 antibody (HA750824) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: JAR cell lysate Lane 4:U-87 MG cell lysate Lane 5: HUVEC cell lysate Lane 6: A375 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: NCI-H226 cell lysate Lane 9: Human liver tissue lysate Lane 10: Human lung tissue lysate Lane 11: Human kidney tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 5 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750824) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MAN2A1 on different lysates with Rabbit anti-MAN2A1 antibody (HA750824) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-MAN2A1 KD cell lysate (10 µg/Lane) Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750824) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MAN2A1 antibody (HA750824) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750824) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750824

Target name :

MAN2A1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MAN2A1 aa 51-500 / 1,144.

Uniprot id :

Q16706>SwissProt: Q16706 Human

Host :

Rabbit

Clone number :

PSH02-67

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, Huh7 cell lysate, JAR cell lysate, U-87 MG cell lysate, HUVEC cell lysate, A375 cell lysate, MDA-MB-468 cell lysate, NCI-H226 cell lysate, Human liver tissue lysate, Human lung tissue lysate, Human kidney tissue lysate, human colon cancer tissue, human stomach tissue, U-87 MG.

Molecular wt :

Predicted band size: 131 kDa

Subcellular location :

Golgi apparatus membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:1,000 ;IHC-P:1:1,000 ;FC:1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750824_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-MAN2A1 antibody (HA750824) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750824) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750824_5.jpg

Pic legend5 :

Flow cytometric analysis of U-87 MG cells labeling MAN2A1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA750824, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage