ERM / Etv5 | HUABIO HA750821 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ERM / Etv5

catalog :

HA750821

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-64

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ERM / Etv5 on different lysates with Rabbit anti-ERM / Etv5 antibody (HA750821) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: HT-29 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: A549 cell lysate Lane 6: HEK-293 cell lysate Lane 7: Hela cell lysate Lane 8: HCT116 cell lysate Lane 9: Neuro-2a cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750821) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ERM / Etv5 antibody (HA750821) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750821) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ERM / Etv5 antibody (HA750821) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750821) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750821

Target name :

ERM / Etv5

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Etv5 aa 1-510 / 510.

Uniprot id :

P41161>SwissProt:P41161 Human;SwissProt:Q9CXC9 Mouse;SwissProt:A0A8I6AQT3 Rat

Host :

Rabbit

Clone number :

PSH02-64

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PC-3M cell lysate, SK-MEL-28 cell lysate, HT-29 cell lysate, SH-SY5Y cell lysate, A549 cell lysate, HEK-293 cell lysate, Hela cell lysate, HCT116 cell lysate, Neuro-2a cell lysate, human brain tissue, human testis tissue, rat brain tissue, SK-MEL-28.

Molecular wt :

Predicted band size: 58 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750821_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ERM / Etv5 antibody (HA750821) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750821) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750821_5.jpg

Pic legend5 :

Immunocytochemistry analysis of SK-MEL-28 cells labeling ERM / Etv5 with Rabbit anti-ERM / Etv5 antibody (HA750821) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERM / Etv5 antibody (HA750821) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750821_6.jpg

Pic legend6 :

Flow cytometric analysis of SK-MEL-28 cells labeling ERM / Etv5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750821, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage