TRIM21 | HUABIO HA750819 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TRIM21

catalog :

HA750819

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-56

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TRIM21 on different lysates with Rabbit anti-TRIM21 antibody (HA750819) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10ng/mL IFN-α for 16 hours cell lysate Lane 3: A549 cell lysate Lane 4: 293T cell lysate Lane 5: U-2 OS cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750819) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of U-2 OS cells labeling TRIM21 with Rabbit anti-TRIM21 antibody (HA750819) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRIM21 antibody (HA750819) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Western blot analysis of TRIM21 on different lysates with Rabbit anti-TRIM21 antibody (HA750819) at 1/5,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si TRIM21 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 45 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750819) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750819

Target name :

TRIM21

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human TRIM21 aa 1-475 (P19474).

Uniprot id :

P19474>SwissProt: P19474 Human

Host :

Rabbit

Clone number :

PSH02-56

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HeLa treated with 10ng/mL IFN-α for 16 hours cell lysate, A549 cell lysate, 293T cell lysate, U-2 OS cell lysate, U-2 OS, human spleen tissue, human tonsil tissue.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Cytoplasm, Cytoplasmic vesicle, autophagosome, Nucleus, P-body.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:10,000 ;IF-Cell: 1:100 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Cell treatment (CT),Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750819_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TRIM21 antibody (HA750819) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750819) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750819_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-TRIM21 antibody (HA750819) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750819) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750819_6.jpg

Pic legend6 :

Flow cytometric analysis of U-2 OS cells labeling TRIM21. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750819, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750819_7.jpg

Pic legend7 :

TRIM21 was immunoprecipitated from 0.2 mg A549 cell lysate with HA750819 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750819 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A549 cell lysate (input) Lane 2: HA750819 IP in A549 cell lysate Lane 3: Rabbit IgG instead of HA750819 in A549 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 6 seconds; ECL: K1801

more info or order :

HUABIO product webpage