NSDHL | HUABIO HA750801 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NSDHL

catalog :

HA750801

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-36

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NSDHL on different lysates with Rabbit anti-NSDHL antibody (HA750801) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate Lane 4: HEK-293 cell lysate Lane 5: AGS cell lysate Lane 6: MCF7 cell lysate Lane 7: MDA-MB-231 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: bEnd.3 cell lysate Lane 10: PC-12 cell lysate Lane 11: C6 cell lysate Lane 12: Human liver tissue lysate Lane 13: Mouse liver tissue lysate Lane 14: Rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 42 kDa Observed band size: 37 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750801) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-NSDHL antibody (HA750801) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750801) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Western blot analysis of NSDHL on different lysates with Rabbit anti-NSDHL antibody (HA750801) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NSDHL KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 40 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750801) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750801

Target name :

NSDHL

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human NSDHL aa 1-300 / 373 (Q15738).

Uniprot id :

Q15738>SwissProt: Q15738 Human;SwissProt: Q9R1J0 Mouse;SwissProt: Q5PPL3 Rat

Host :

Rabbit

Clone number :

PSH02-36

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, HEK-293 cell lysate, AGS cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, NIH/3T3 cell lysate, bEnd.3 cell lysate, PC-12 cell lysate, C6 cell lysate, human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human colon cancer tissue, human liver cancer tissue, human liver tissue, human stomach cancer tissue, mouse liver tissue, rat liver tissue, HepG2, NIH/3T3.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Endoplasmic reticulum membrane, Lipid droplet

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IF-Cell: 1:100 ;FC: 1:100-1:1,000 ;IP: 1-2μg/sample

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750801_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-NSDHL antibody (HA750801) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750801) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750801_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NSDHL antibody (HA750801) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750801) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750801_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-NSDHL antibody (HA750801) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750801) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750801_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-NSDHL antibody (HA750801) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750801) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750801_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-NSDHL antibody (HA750801) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750801) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750801_9.jpg

Pic legend9 :

Immunocytochemistry analysis of HepG2 cells labeling NSDHL with Rabbit anti-NSDHL antibody (HA750801) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NSDHL antibody (HA750801) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750801_10.jpg

Pic legend10 :

Immunocytochemistry analysis of NIH/3T3 cells labeling NSDHL with Rabbit anti-NSDHL antibody (HA750801) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NSDHL antibody (HA750801) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750801_11.jpg

Pic legend11 :

Flow cytometric analysis of HepG2 cells labeling NSDHL. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750801, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA750801_12.jpg

Pic legend12 :

Flow cytometric analysis of NIH/3T3 cells labeling NSDHL. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750801, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA750801_13.jpg

Pic legend13 :

NSDHL was immunoprecipitated from 0.2 mg HeLa cell lysate with HA750801 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750801 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of HA750801 in HeLa cell lysate Lane 3: HA750801 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL: K1801

more info or order :

HUABIO product webpage