product summary
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company name :
HUABIO
product type :
antibody
product name :
TXNRD2
catalog :
HA750794
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH02-26
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of TXNRD2 on different lysates with Rabbit anti-TXNRD2 antibody (HA750794) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat heart tissue lysate (40 µg/Lane)
Lane 7: Mouse liver tissue lysate (40 µg/Lane)
Lane 8: Rat liver tissue lysate (40 µg/Lane)
Lane 9: Human kidney tissue lysate (40 µg/Lane)
Lane 10: Mouse kidney tissue lysate (40 µg/Lane)
Lane 11: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750794) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-TXNRD2 antibody (HA750794) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750794) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-TXNRD2 antibody (HA750794) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750794) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA750794
Target name :
TXNRD2
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human TXNRD2 aa 1-524 / 524.
Uniprot id :
Q9NNW7>SwissProt: Q9NNW7 Human;SwissProt: Q9JLT4 Mouse;SwissProt: Q9Z0J5 Rat
Host :
Rabbit
Clone number :
PSH02-26
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HEK-293 cell lysate, HeLa cell lysate, A431 cell lysate, K-562 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human kidney tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human prostate tissue, human liver tissue, mouse liver tissue, rat liver tissue.
Molecular wt :
Predicted band size: 57 kDa
Subcellular location :
Mitochondrion
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:200-1:1,000
;IF-Tissue: 1:50
Advanced Validation :
Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750794_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-TXNRD2 antibody (HA750794) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750794) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA750794_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-TXNRD2 antibody (HA750794) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750794) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA750794_6.jpg
Pic legend6 :
Immunofluorescence analysis of paraffin-embedded rat liver tissue labeling TXNRD2 with Rabbit anti-TXNRD2 antibody (HA750794) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750794, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA750794_7.jpg
Pic legend7 :
Western blot analysis of TXNRD2 on different lysates with Rabbit anti-TXNRD2 antibody (HA750794) at 1/1,000 dilution.
Lane 1: MCF7-si NT cell lysate
Lane 2: MCF7-si TXNRD2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750794) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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