TRIM56 | HUABIO HA750787 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TRIM56

catalog :

HA750787

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-17

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA750787) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: 293T cell lysate Lane 5: A375 cell lysate Lane 6: COS-1 cell lysate Lane 7: C2C12 cell lysate Lane 8: PC-12 cell lysate Lane 9: Mouse lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750787) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of TRIM56 on different lysates with Rabbit anti-TRIM56 antibody (HA750787) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate (20 µg/Lane) Lane 2: A549-si TRIM56 cell lysate (20 µg/Lane) Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750787) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Flow cytometric analysis of HeLa cells labeling TRIM56. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750787, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA750787

Target name :

TRIM56

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human TRIM56 aa 350-755 (Q9BRZ2).

Uniprot id :

Q9BRZ2>SwissProt: Q9BRZ2 Human;SwissProt: Q80VI1 Mouse;SwissProt: A0A8I6G8D1 Rat

Host :

Rabbit

Clone number :

PSH02-17

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, 293T cell lysate, A375 cell lysate, COS-1 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse lung tissue lysate, HeLa, NIH/3T3, C2C12, PC-12.

Molecular wt :

Predicted band size: 81 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;FC: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750787_4.jpg

Pic legend4 :

Flow cytometric analysis of NIH/3T3 cells labeling TRIM56. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750787, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750787_5.jpg

Pic legend5 :

Flow cytometric analysis of C2C12 cells labeling TRIM56. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750787, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750787_6.jpg

Pic legend6 :

Flow cytometric analysis of PC-12 cells labeling TRIM56. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750787, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage