F4/80 | HUABIO HA750761 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

F4/80

catalog :

HA750761

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-87

reactivity :

mouse

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA750761) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 2: L-929 cell lysate (no heat) (negative) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750761) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (HA750761) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750761) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (HA750761) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750761) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750761

Target name :

F4/80

Species reactivity :

Mouse

Applications :

WB,IHC-P,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse F4/80 aa 1-650 / 931.

Uniprot id :

Q61549>SwissProt: Q61549 Mouse

Host :

Rabbit

Clone number :

PSH01-87

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

RAW264.7 cell lysate, mouse liver tissue, mouse spleen tissue, mouse lung tissue.

Molecular wt :

Predicted band size: 102 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:5,000 ;IF-Tissue: 1:500 ;IHC-Fr: 1:500-1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750761_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-F4/80 antibody (HA750761) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750761) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750761_5.jpg

Pic legend5 :

Application: IHC-Fr Species: Mouse Site: Liver Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750761_6.jpg

Pic legend6 :

Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750761_7.jpg

Pic legend7 :

Application: IF-tissue Species: Mouse Site: Liver Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750761_8.jpg

Pic legend8 :

Application: IF-tissue Species: Mouse Site: Spleen Sample: Paraffin-embedded section Antibody concentration: 1/500

more info or order :

HUABIO product webpage