product summary
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company name :
HUABIO
product type :
antibody
product name :
FXR1
catalog :
HA750743
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH01-67
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (HA750743) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A549 cell lysate
Lane 5: K-562 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: Mouse skeletal muscle tissue lysate
Lane 11: Rat skeletal muscle tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70/75 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750743) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/10,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FXR1 antibody (HA750743) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750743) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-FXR1 antibody (HA750743) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750743) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA750743
Target name :
FXR1
Species reactivity :
Human,Mouse,Rat,Monkey
Applications :
WB,IHC-P,IF-Cell,FC,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human FXR1 aa 1-150 / 621.
Uniprot id :
P51114>SwissProt: P51114 Human;SwissProt: Q61584 Mouse;SwissProt: Q5XI81 Rat
Host :
Rabbit
Clone number :
PSH01-67
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, 293T cell lysate, HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human brain tissue, human testis tissue, mouse brain tissue, rat brain tissue, PC-12, NIH/3T3.
Molecular wt :
Predicted band size: 70 kDa
Subcellular location :
Cytoplasm, Cytoplasmic ribonucleoprotein granule, Stress granule, Cell projection, dendrite, dendritic spine, axon, Nucleus envelope, Postsynapse.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:1,000-1:3,000
;IF-Cell: 1:100
;FC: 1:100
;IP: 1-2μg/sample
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750743_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-FXR1 antibody (HA750743) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750743) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA750743_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-FXR1 antibody (HA750743) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750743) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA750743_6.jpg
Pic legend6 :
Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody (HA750743) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (HA750743) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA750743_7.jpg
Pic legend7 :
Flow cytometric analysis of NIH/3T3 cells labeling FXR1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750743, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA750743_8.jpg
Pic legend8 :
FXR1 was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA750743 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750743 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (input)
Lane 2: HA750743 IP in HepG2 cell lysate
Lane 3: Rabbit IgG instead of HA750743 in HepG2 cell lysate
Blocking/Dilution buffer: primary antibody dilution (K1803)
Exposure time: 2 seconds; ECL: K1801
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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