Otx2 | HUABIO HA750737 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Otx2

catalog :

HA750737

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-61

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: E14.5 embryo Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Otx2 antibody (HA750737) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750737) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

product information

SKU :

HA750737

Target name :

Otx2

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Otx2 aa 1-289.

Uniprot id :

P32243>SwissProt: P32243 Human;SwissProt: P80206 Mouse;SwissProt: Q64201 Rat

Host :

Rabbit

Clone number :

PSH01-61

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

293T transfected with FLAG-tagged Otx2 cell lysate, mouse eyeball tissue lysate, rat embryo tissue lysate, rat eyeball tissue lysate, mouse embryo tissue, mouse eye tissue, rat eye tissue, 293T overexpress with Otx2, E14.5 mouse embryo tissue.

Molecular wt :

Predicted band size: 32 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:200-1:1,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:200 ;IF-Cell: 1:10,000 ;FC: 1:1,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750737_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse eye tissue with Rabbit anti-Otx2 antibody (HA750737) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750737) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750737_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat eye tissue with Rabbit anti-Otx2 antibody (HA750737) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750737) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750737_6.jpg

Pic legend6 :

Western blot analysis of Otx2 on different lysates with Rabbit anti-Otx2 antibody (HA750737) at 1/1,000 dilution. Lane 1: 293T transfected with FLAG-tagged empty control cell lysate Lane 2: 293T transfected with FLAG-tagged Otx2 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 32 kDa Observed band size: 35 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750737) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750737_7.jpg

Pic legend7 :

Western blot analysis of Otx2 on different lysates with Rabbit anti-Otx2 antibody (HA750737) at 1/1,000 dilution. Lane 1: Mouse eyeball tissue lysate (40 µg/Lane) Lane 2: Rat embryo tissue lysate (40 µg/Lane) Lane 3: Rat eyeball tissue lysate (40 µg/Lane) Predicted band size: 32 kDa Observed band size: 35 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750737) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750737_8.jpg

Pic legend8 :

Western blot analysis of Otx2 on Y79 cell lysates with Rabbit anti-Otx2 antibody (HA750737) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 35 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750737_9.jpg

Pic legend9 :

Application: IF-tissue Species: Mouse Site: E14.5 embryo Sample: Paraffin-embedded section Antibody concentration: 1:500

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750737_10.jpg

Pic legend10 :

Immunocytochemistry analysis of 293T overexpress with or without Otx2 cells labeling Otx2 with Rabbit anti-Otx2 antibody (HA750737) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Otx2 antibody (HA750737) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750737_11.jpg

Pic legend11 :

Flow cytometric analysis of 293T overexpress with or without Otx2 cells labeling Otx2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750737, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage