product summary
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company name :
HUABIO
product type :
antibody
product name :
TMP21
catalog :
HA750718
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH01-40
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of TMP21 on different lysates with Rabbit anti-TMP21 antibody (HA750718) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: Ramos cell lysate (20 µg/Lane)
Lane 7: U-87 MG cell lysate (20 µg/Lane)
Lane 8: RAW264.7 cell lysate (20 µg/Lane)
Lane 9: Neuro-2a cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse pancreas tissue lysate (40 µg/Lane)
Lane 12: Mouse liver tissue lysate (40 µg/Lane)
Lane 13: Rat pancreas tissue lysate (40 µg/Lane)
Lane 14: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 19 kDa
Exposure time: 2 minutes 13 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750718) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-TMP21 antibody (HA750718) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750718) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-TMP21 antibody (HA750718) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750718) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA750718
Target name :
TMP21
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human TMP21 aa 1-200 / 219.
Uniprot id :
P49755>SwissProt: P49755 Human;SwissProt: Q9D1D4 Mouse;SwissProt: Q63584 Rat
Host :
Rabbit
Clone number :
PSH01-40
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, 293T cell lysate, Raji cell lysate, Jurkat cell lysate, HepG2 cell lysate, Ramos cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse pancreas tissue lysate, mouse liver tissue lysate, rat pancreas tissue lysate, rat liver tissue lysate, human breast tissue, human endometrium tissue, mouse pancreas tissue, rat pancreas tissue.
Molecular wt :
Predicted band size: 25 kDa
Subcellular location :
Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Golgi apparatus, cis-Golgi network membrane, Golgi apparatus, trans-Golgi network membrane, Cytoplasmic vesicle, secretory vesicle membrane, Cell membrane, Melanosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000
;IHC-P: 1:200-1:1,000
;IF-Tissue: 1:200
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750718_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-TMP21 antibody (HA750718) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750718) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA750718_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-TMP21 antibody (HA750718) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750718) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA750718_6.jpg
Pic legend6 :
Immunofluorescence analysis of paraffin-embedded human breast tissue labeling TMP21 with Rabbit anti-TMP21 antibody (HA750718) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750718, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA750718_7.jpg
Pic legend7 :
Immunofluorescence analysis of paraffin-embedded rat pancreas tissue labeling TMP21 with Rabbit anti-TMP21 antibody (HA750718) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750718, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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