product summary
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company name :
HUABIO
product type :
antibody
product name :
Phospho-PI3K p85 (Y467) + PI3K p55 (Y199)
catalog :
HA750717
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH01-39
reactivity :
human, mouse
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA750717 image 1
Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody (HA750717) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750717) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA750717 image 2
Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody (HA750717) at 1/1,000 dilution. Lane 1: 293T overexpress Src whole cell lysate Lane 2: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lane 3: 293T overexpress Src treated with λpp for 1 hour whole cell lysate Lane 4: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes, then treated with λpp for 1 hour whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 55 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750717) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA750717
Target name :
Phospho-PI3K p85 (Y467) + PI3K p55 (Y199)
Species reactivity :
Human,Mouse
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human PIK3R1 aa 451-500 / 724.
Uniprot id :
P27986>SwissProt: P27986 Human;SwissProt: P26450 Mouse
Host :
Rabbit
Clone number :
PSH01-39
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate.
Molecular wt :
Predicted band size: 84 kDa/55 kDa
Subcellular location :
Cell-cell junction, cis-Golgi network, cytoplasm, cytosol, membrane, nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;IF-Cell: 1:100
Advanced Validation :
Cell treatment (CT)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!