Ubiquinol-Cytochrome C Reductase Core Protein I | HUABIO HA750699 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ubiquinol-Cytochrome C Reductase Core Protein I

catalog :

HA750699

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-23

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Ubiquinol-Cytochrome C Reductase Core Protein I on different lysates with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: PC-3M cell lysate (20 µg/Lane) Lane 6: Jurkat cell lysate (20 µg/Lane) Lane 7: NIH/3T3 cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: Mouse kidney tissue lysate (40 µg/Lane) Lane 10: Mouse brain tissue lysate (40 µg/Lane) Lane 11: Rat kidney tissue lysate (40 µg/Lane) Lane 12: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 53 kDa Observed band size: 45 kDa Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750699) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750699) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750699) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750699

Target name :

Ubiquinol-Cytochrome C Reductase Core Protein I

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human UQCRC1 aa 1-480 / 480.

Uniprot id :

P31930>SwissProt: P31930 Human;SwissProt: Q9CZ13 Mouse;SwissProt: Q68FY0 Rat

Host :

Rabbit

Clone number :

PSH01-23

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, HepG2 cell lysate, HCT 116 cell lysate, 293T cell lysate, PC-3M cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, mouse brain tissue lysate, rat kidney tissue lysate, rat brain tissue lysate, human kidney tissue, human liver tissue, human small intestine tissue, mouse kidney tissue, HepG2, 293T.

Molecular wt :

Predicted band size: 53 kDa

Subcellular location :

Mitochondrion inner membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:5,000 ;IF-Tissue: 1:1,000 ;IF-Cell: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750699_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750699) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750699_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750699) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750699_6.jpg

Pic legend6 :

Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750699, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750699_7.jpg

Pic legend7 :

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750699, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750699_8.jpg

Pic legend8 :

Immunocytochemistry analysis of HepG2 cells labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750699_9.jpg

Pic legend9 :

Immunocytochemistry analysis of 293T cells labeling Ubiquinol-Cytochrome C Reductase Core Protein I with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody (HA750699) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage