PPP2R5D | HUABIO HA750697 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PPP2R5D

catalog :

HA750697

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-21

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (HA750697) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: 293T cell lysate (20 µg/Lane) Lane 6: Jurkat cell lysate (20 µg/Lane) Lane 7: Human brain tissue lysate (40 µg/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750697) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (HA750697) at 1/1,000 dilution. Lane 1: Human PPP2R5C recombinant protein aa (aa1-524) 50ng Lane 2: Human PPP2R5D recombinant protein aa (aa107-602) 50ng Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750697) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of A431 cells labeling PPP2R5D with Rabbit anti-PPP2R5D antibody (HA750697) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R5D antibody (HA750697) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750697

Target name :

PPP2R5D

Species reactivity :

Human

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PPP2R5D aa 107-602 / 602.

Uniprot id :

Q14738>SwissProt: Q14738 Human

Host :

Rabbit

Clone number :

PSH01-21

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, 293T cell lysate, Jurkat cell lysate, human brain tissue lysate, A431.

Molecular wt :

Predicted band size: 70 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:500-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750697_4.jpg

Pic legend4 :

Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750697, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage