product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
IL-18
catalog :
HA750677
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PS01-06
reactivity :
human
application :
western blot, flow cytometry
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA750677 image 1
Western blot analysis of IL-18 on different lysates with Rabbit anti-IL-18 antibody (HA750677) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750677) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA750677 image 2
Flow cytometric analysis of A431 cells labeling IL-18. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750677, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA750677
Target name :
IL-18
Species reactivity :
Human
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within full length protein.
Uniprot id :
Q14116>SwissProt: Q14116 Human
Host :
Rabbit
Clone number :
PS01-06
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
A431 cell lysate, SiHa cell lysate, A431.
Molecular wt :
Predicted band size: 22 kDa
Subcellular location :
Cytoplasm, Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;FC: 1:500-1:1,000
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!