product summary
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company name :
HUABIO
product type :
antibody
product name :
F4/80
catalog :
HA750665
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH0-85
reactivity :
mouse, rat
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA750665 image 1
Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA750665) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate (no heat) (40 µg/Lane) Lane 2: Mouse kidney tissue lysate (40 µg/Lane) Lane 3: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 4: Rat kidney tissue lysate (no heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750665) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA750665 image 2
Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA750665) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane) Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane) Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750665) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 3 :
HUABIO HA750665 image 3
Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 (F4/80, HA750665, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green) Antigen retrieval: Not required
product information
SKU :
HA750665
Target name :
F4/80
Species reactivity :
Mouse,Rat
Applications :
WB,IHC-P,IF-Tissue,IHC-Fr
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse F4/80 aa 1-650 / 931.
Uniprot id :
Q61549>SwissProt: Q61549 Mouse;SwissProt: Q5Y4N8 Rat
Host :
Rabbit
Clone number :
PSH0-85
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse kidney tissue lysate, RAW264.7 cell lysate, Rat kidney tissue lysate, Rat spleen tissue lysate, mouse liver tissue, mouse spleen tissue, rat liver tissue, rat spleen tissue, mouse lung tissue, rat lung tissue.
Molecular wt :
Predicted band size: 102 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IF-Tissue: 1:500 ;IHC-Fr: 1:1,000
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750665_4.jpg
Pic legend4 :
Application: IHC-Fr Species: Rat Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 (F4/80, HA750665, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green) Antigen retrieval: Not required
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA750665_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (HA750665) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750665) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA750665_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (HA750665) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750665) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA750665_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-F4/80 antibody (HA750665) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750665) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA750665_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-F4/80 antibody (HA750665) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750665) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/HA750665_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-F4/80 antibody (HA750665) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750665) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img10 :
https://storage.huabio.cn/huabio/productImg/HA750665_10.jpg
Pic legend10 :
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-F4/80 antibody (HA750665) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750665) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img11 :
https://storage.huabio.cn/huabio/productImg/HA750665_11.jpg
Pic legend11 :
Application: IF-tissue Species: Mouse Site: Liver Sample: Paraffin-embedded section Antibody concentration: 1/500
Pic img12 :
https://storage.huabio.cn/huabio/productImg/HA750665_12.jpg
Pic legend12 :
Application: IF-tissue Species: Rat Site: Liver Sample: Paraffin-embedded section Antibody concentration: 1/500
Pic img13 :
https://storage.huabio.cn/huabio/productImg/HA750665_13.jpg
Pic legend13 :
Application: IF-tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/500
Pic img14 :
https://storage.huabio.cn/huabio/productImg/HA750665_14.jpg
Pic legend14 :
Application: IF-tissue Species: Mouse Site: small intestine Sample: Paraffin-embedded section Antibody concentration: 1/500
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!