Pancreatic Polypeptide | HUABIO HA750664 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Pancreatic Polypeptide

catalog :

HA750664

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-84

reactivity :

human, mouse, rat

application :

immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of frozen mouse pancreas tissue with Rabbit anti-Pancreatic Polypeptide antibody (HA750664) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750664, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). Insulin (EM80714, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunofluorescence analysis of frozen rat pancreas tissue with Rabbit anti-Pancreatic Polypeptide antibody (HA750664) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750664, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). Insulin (EM80714, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

product information

SKU :

HA750664

Target name :

Pancreatic Polypeptide

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-P,IHC-Fr,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PPY aa 30-95.

Uniprot id :

P01298>SwissProt: P01298 Human;SwissProt: P10601 Mouse;SwissProt: P06303 Rat

Host :

Rabbit

Clone number :

PSH0-84

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse pancreas tissue, rat pancreas tissue.

Molecular wt :

Predicted band size: 10 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:1,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:50

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750664_4.jpg

Pic legend4 :

Immunofluorescence analysis of frozen mouse liver tissue (negative) with Rabbit anti-Pancreatic Polypeptide antibody (HA750664) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750664, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750664_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Pancreatic Polypeptide antibody (HA750664) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750664) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750664_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Pancreatic Polypeptide antibody (HA750664) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750664) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage