Lipoamide Dehydrogenase | HUABIO HA750663 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Lipoamide Dehydrogenase

catalog :

HA750663

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-83

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Lipoamide Dehydrogenase on different lysates with Rabbit anti-Lipoamide Dehydrogenase antibody (HA750663) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: SK-Br-3 cell lysate (20 µg/Lane) Lane 7: U-937 cell lysate (20 µg/Lane) Lane 8: HepG2 cell lysate (20 µg/Lane) Lane 9: NIH/3T3 cell lysate (20 µg/Lane) Lane 10: Mouse liver tissue lysate (40 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750663) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Lipoamide Dehydrogenase antibody (HA750663) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750663) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lipoamide Dehydrogenase antibody (HA750663) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750663) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750663

Target name :

Lipoamide Dehydrogenase

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Lipoamide Dehydrogenase 21-509 / 509.

Uniprot id :

P09622>SwissProt: P09622 Human;SwissProt: O08749 Mouse

Host :

Rabbit

Clone number :

PSH0-83

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, K-562 cell lysate, SK-Br-3 cell lysate, U-937 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, human colon tissue, human colon carcinoma tissue, HeLa.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Mitochondrion matrix, Nucleus, Cell projection, cilium, flagellum, Cytoplasmic vesicle, secretory vesicle, acrosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:5,000 ;IF-Cell: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750663_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HeLa cells labeling Lipoamide Dehydrogenase with Rabbit anti-Lipoamide Dehydrogenase antibody (HA750663) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lipoamide Dehydrogenase antibody (HA750663) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

more info or order :

HUABIO product webpage