AIMP2 | HUABIO HA750654 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

AIMP2

catalog :

HA750654

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-75

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of AIMP2 on different lysates with Rabbit anti-AIMP2 antibody (HA750654) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: HepG2 cell lysate Lane 6: HT-29 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse kidney tissue lysate (40 µg/Lane) Lane 9: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750654) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-AIMP2 antibody (HA750654) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750654) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunocytochemistry analysis of SH-SY5Y cells labeling AIMP2 with Rabbit anti-AIMP2 antibody (HA750654) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-AIMP2 antibody (HA750654) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750654

Target name :

AIMP2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human AIMP2 aa 81-320 / 320.

Uniprot id :

Q13155>SwissProt: Q13155 Human;SwissProt: Q8R010 Mouse;SwissProt: Q32PX2 Rat

Host :

Rabbit

Clone number :

PSH0-75

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, HT-29 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human testis tissue, SH-SY5Y, A549.

Molecular wt :

Predicted band size: 35 kDa

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:500-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750654_4.jpg

Pic legend4 :

Immunocytochemistry analysis of A549 cells labeling AIMP2 with Rabbit anti-AIMP2 antibody (HA750654) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-AIMP2 antibody (HA750654) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750654_5.jpg

Pic legend5 :

Flow cytometric analysis of SH-SY5Y cells labeling AIMP2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750654, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750654_6.jpg

Pic legend6 :

Flow cytometric analysis of A549 cells labeling AIMP2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750654, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage