Cystathionase | HUABIO HA750649 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cystathionase

catalog :

HA750649

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE35-91

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (HA750649) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Lane 5: NCI-H441 cell lysate (20 µg/Lane) Lane 6: A431 cell lysate (20 µg/Lane) Lane 7: F9 cell lysate (20 µg/Lane) Lane 8: Human liver tissue lysate (40 µg/Lane) Lane 9: Mouse liver tissue lysate (40 µg/Lane) Lane 10: Rat liver tissue lysate (40 µg/Lane) Lane 11: Human kidney tissue lysate (40 µg/Lane) Lane 12: Mouse kidney tissue lysate (40 µg/Lane) Lane 13: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750649) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Cystathionase was immunoprecipitated in 0.2mg A431 cell lysate with HA750649 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750649 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA750649 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA750649 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds

image 3 :

Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (HA750649) at 1/10,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Cystathionase KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750649) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750649

Target name :

Cystathionase

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Cystathionase aa 1-405 / 405.

Uniprot id :

P32929>SwissProt: P32929 Human;SwissProt: Q8VCN5 Mouse;SwissProt: P18757 Rat

Host :

Rabbit

Clone number :

JE35-91

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, 293T cell lysate, K-562 cell lysate, HEK-293 cell lysate, NCI-H441 cell lysate, A431 cell lysate, F9 cell lysate, human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human kidney tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:10,000 ;IP: 1-2μg/sample

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage