product summary
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company name :
HUABIO
product type :
antibody
product name :
SMC2
catalog :
HA750639
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH0-62
reactivity :
human
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (HA750639) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (30 µg/Lane)
Lane 2: HEK-293 cell lysate (30 µg/Lane)
Lane 3: HT-29 cell lysate (30 µg/Lane)
Lane 4: HepG2 cell lysate (30 µg/Lane)
Lane 5: A431 cell lysate (30 µg/Lane)
Lane 6: SH-SY5Y cell lysate (30 µg/Lane)
Lane 7: Jurkat cell lysate (30 µg/Lane)
Lane 8: Raji cell lysate (30 µg/Lane)
Lane 9: COS-1 cell lysate (24 µg/Lane)
Predicted band size: 136 kDa
Observed band size: 136 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750639) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMC2 antibody (HA750639) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750639) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SMC2 antibody (HA750639) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750639) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA750639
Target name :
SMC2
Species reactivity :
Human,Monkey
Applications :
WB,IHC-P,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human SMC2 aa 451-750 / 1,197.
Uniprot id :
O95347>SwissProt: O95347 Human
Host :
Rabbit
Clone number :
PSH0-62
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, HEK-293 cell lysate, HT-29 cell lysate, HepG2 cell lysate, A431 cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, Raji cell lysate, COS-1 cell lysate, human colon carcinoma tissue, human colon tissue, human colon lymph nodes tissue, HeLa, HT-29.
Molecular wt :
Predicted band size: 136 kDa
Subcellular location :
Nucleus, Cytoplasm, Chromosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000;IHC-P: 1:1,000;IF-Cell: 1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750639_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human colon lymph nodes tissue with Rabbit anti-SMC2 antibody (HA750639) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750639) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA750639_5.jpg
Pic legend5 :
Immunocytochemistry analysis of HeLa cells labeling SMC2 with Rabbit anti-SMC2 antibody (HA750639) at 1/100 dilution.
Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (HA750639) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA750639_6.jpg
Pic legend6 :
Immunocytochemistry analysis of HT-29 cells labeling SMC2 with Rabbit anti-SMC2 antibody (HA750639) at 1/100 dilution.
Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (HA750639) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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