Mesothelin | HUABIO HA750634 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Mesothelin

catalog :

HA750634

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-57

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Mesothelin on different lysates with Rabbit anti-Mesothelin antibody (HA750634) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: OVCAR-3 cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 45 kDa Exposure time: 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750634) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue with Rabbit anti-Mesothelin antibody (HA750634) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750634) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue with Rabbit anti-Mesothelin antibody (HA750634) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750634) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750634

Target name :

Mesothelin

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Mesothelin aa 531-580 / 622 (Q13421-3).

Uniprot id :

Q13421>SwissProt: Q13421 Human

Host :

Rabbit

Clone number :

PSH0-57

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, SK-OV-3 cell lysate, OVCAR-3 cell lysate, SiHa cell lysate, NCI-H226 cell lysate, PC-3M cell lysate, human mesothelioma tissue, human ovarian cancer tissue, human tonsil tissue.

Molecular wt :

Predicted band size: 69 kDa

Subcellular location :

Cell membrane, Golgi apparatus, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:500-1:2,000 ;IF-Tissue: 1:100

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750634_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Mesothelin antibody (HA750634) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750634) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750634_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (negative) with Rabbit anti-Mesothelin antibody (HA750634) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750634) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750634_6.jpg

Pic legend6 :

Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Mesothelin with Rabbit anti-Mesothelin antibody (HA750634) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750634, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750634_7.jpg

Pic legend7 :

Immunofluorescence analysis of paraffin-embedded human ovarian cancer tissue labeling Mesothelin with Rabbit anti-Mesothelin antibody (HA750634) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750634, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage