product summary
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company name :
HUABIO
product type :
antibody
product name :
LXR beta
catalog :
HA750633
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH0-56
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of LXR beta on different lysates with Rabbit anti-LXR beta antibody (HA750633) at 1/1,000 dilution.
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: PC-3M cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: HeLa cell lysate (20 µg/Lane)
Lane 6: HT-29 cell lysate (20 µg/Lane)
Lane 7: HCT116 cell lysate (20 µg/Lane)
Lane 8: HEK-293 cell lysate (20 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)
Lane 10: Mouse liver tissue lysate (40 µg/Lane)
Lane 11: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750633) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of C2C12 cells labeling LXR beta with Rabbit anti-LXR beta antibody (HA750633) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LXR beta antibody (HA750633) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Immunocytochemistry analysis of HCT116 cells labeling LXR beta with Rabbit anti-LXR beta antibody (HA750633) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LXR beta antibody (HA750633) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
HA750633
Target name :
LXR beta
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human LXR beta aa 50-100/460.
Uniprot id :
P55055>SwissProt: P55055 Human;SwissProt: Q60644 Mouse;SwissProt: Q62755 Rat
Host :
Rabbit
Clone number :
PSH0-56
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SW480 cell lysate, HepG2 cell lysate, PC-3M cell lysate, A549 cell lysate, HeLa cell lysate, HT-29 cell lysate, HCT116 cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, C2C12, HCT116.
Molecular wt :
Predicted band size: 51 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IF-Cell: 1:100
Advanced Validation :
Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA750633_4.jpg
Pic legend4 :
Western blot analysis of LXR beta on different lysates with Rabbit anti-LXR beta antibody (HA750633) at 1/1,000 dilution.
Lane 1: HEK-293-si NT cell lysate
Lane 2: HEK-293-si LXR beta cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750633) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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