Tankyrase 2 | HUABIO HA750630 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Tankyrase 2

catalog :

HA750630

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-53

reactivity :

human, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/1,000 dilution. Lane 1: PC-12 whole cell lysate Lane 2: PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate Lane 3: PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750630) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Tankyrase 2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 127 kDa Observed band size: 90 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750630) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tankyrase 2 antibody (HA750630) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750630

Target name :

Tankyrase 2

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Tankyrase 2 aa 451-700/1,166.

Uniprot id :

Q9H2K2>SwissProt: Q9H2K2 Human;Entrez Gene: 309512 Rat

Host :

Rabbit

Clone number :

PSH0-53

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate, PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate, A431.

Molecular wt :

Predicted band size: 127 kDa

Subcellular location :

Cytoplasm, Golgi apparatus membrane, Nucleus, Chromosome, telomere.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:250

Advanced Validation :

Cell treatment (CT),Knockdown (KD)

more info or order :

HUABIO product webpage