AKT1 | HUABIO HA750627 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

AKT1

catalog :

HA750627

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-50

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (HA750627) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: COS-1 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750627) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of Jurkat cells labeling AKT1 with Rabbit anti-AKT1 antibody (HA750627) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-AKT1 antibody (HA750627) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Flow cytometric analysis of Jurkat cells labeling AKT1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750627, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA750627

Target name :

AKT1

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human AKT1 aa 271-320 / 480.

Uniprot id :

P31749>SwissProt: P31749 Human;SwissProt: P31750 Mouse;SwissProt: P47196 Rat

Host :

Rabbit

Clone number :

PSH0-50

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, COS-1 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, Jurkat.

Molecular wt :

Predicted band size: 55.7 kDa

Subcellular location :

Cytoplasm, Nucleus, Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50 ;FC: 1:50-1:1,000

more info or order :

HUABIO product webpage