NDP52 | HUABIO HA750623 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NDP52

catalog :

HA750623

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-33

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NDP52 on different lysates with Rabbit anti-NDP52 antibody (HA750623) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lane 5: Huh7 cell lysate Lane 6: HepG2 cell lysate Lane 7: Saos-2 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750623) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of HeLa cells labeling NDP52. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750623, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA750623

Target name :

NDP52

Species reactivity :

Human,Mouse

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human NDP52 aa 1-150 / 446 (Q13137).

Uniprot id :

Q13137>SwissProt: Q13137 Human

Host :

Rabbit

Clone number :

PSH02-33

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Immunogen affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, THP-1 cell lysate, Huh7 cell lysate, HepG2 cell lysate, Saos-2 cell lysate, RAW264.7 cell lysate, HeLa.

Molecular wt :

Predicted band size: 52 kDa

Subcellular location :

Cytoplasm, perinuclear region, cytoskeleton, cytoplasmic vesicle, autophagosome membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;FC: 1:1,000

more info or order :

HUABIO product webpage