product summary
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company name :
HUABIO
product type :
antibody
product name :
GPD2
catalog :
HA750605
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH0-31
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (HA750605) at 1/1,000 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U87-MG cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 81 kDa
Observed band size: 68 kDa
Exposure time: 10s;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750605) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 2 :
Flow cytometric analysis of Hela cells labeling GPD2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA750605, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA750605
Target name :
GPD2
Species reactivity :
Human
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human GPD2 aa 43-727.
Uniprot id :
P4$3304>SwissProt: P4$3304 Human
Host :
Rabbit
Clone number :
PSH0-31
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
MCF-7 cell lysate, THP-1 cell lysate, U87-MG cell lysate, HeLa cell lysate, Hela cell.
Molecular wt :
Predicted band size: 81 kDa
Subcellular location :
Mitochondrion
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;FC:1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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