NF-kB p65 | HUABIO HA750601 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NF-kB p65

catalog :

HA750601

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-27

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/500 dilution. Lane 1: HeLa cell lysate (control) (35 µg/Lane) Lane 2: HeLa-RELA-KO (1) (35 µg/Lane) Lane 3: HeLa-RELA-KO (2) (35 µg/Lane) Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750601) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/500 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: PC-12 cell lysate (10 µg/Lane) Lane 4: A431 cell lysate (10 µg/Lane) Lane 5: Raji cell lysate (10 µg/Lane) Lane 6: HL-60 cell lysate (10 µg/Lane) Lane 7: Daudi cell lysate (10 µg/Lane) Lane 8: COS-1 cell lysate (10 µg/Lane) Lane 9: Mouse lung tissue lysate (20 µg/Lane) Predicted band size: 60 kDa Observed band size: 65 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750601) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750601

Target name :

NF-kB p65

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human RELA aa 502-551 / 551.

Uniprot id :

Q04206>SwissProt: Q04206 Human;SwissProt: Q04207 Mouse;Entrez Gene: 309165 Rat

Host :

Rabbit

Clone number :

PSH0-27

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, A431 cell lysate, Raji cell lysate, HL-60 cell lysate, Daudi cell lysate, COS-1 cell lysate, Mouse lung tissue lysate, human breast cancer tissue, human breast tissue, HeLa, NIH/3T3 cells treated with 50μg/mL TNF-α for 20 minutes, PC-12.

Molecular wt :

Predicted band size: 65 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100-1:200 ;FC: 1:1,000

Advanced Validation :

Knockout (KO),Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750601_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750601_5.jpg

Pic legend5 :

Immunocytochemistry analysis of HeLa cells labeling NF-kB p65 with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750601_6.jpg

Pic legend6 :

Immunocytochemistry analysis of NIH/3T3 cells treated with or without 50μg/mL TNF-α for 20 minutes labeling NF-kB p65 with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NF-kB p65 antibody (HA750601) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750601_7.jpg

Pic legend7 :

Flow cytometric analysis of HeLa cells labeling NF-kB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750601, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750601_8.jpg

Pic legend8 :

Flow cytometric analysis of NIH/3T3 cells labeling NF-kB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750601, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750601_9.jpg

Pic legend9 :

Flow cytometric analysis of PC-12 cells labeling NF-kB p65. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750601, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage