PBX1 | HUABIO HA750598 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PBX1

catalog :

HA750598

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-24

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PBX1 on different lysates with Rabbit anti-PBX1 antibody (HA750598) at 1/1,000 dilution. Lane 1: Hela cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: MCF-7 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Lane 8: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 47 kDa Observed band size: 40/47 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750598) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PBX1 antibody (HA750598) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750598) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-PBX1 antibody (HA750598) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750598) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750598

Target name :

PBX1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PBX1 aa 1-250 / 430.

Uniprot id :

P40424>SwissProt: P40424 Human;SwissProt: P41778 Mouse;Entrez Gene: 304947 Rat

Host :

Rabbit

Clone number :

PSH0-24

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela cell lysate, HEK-293 cell lysate, MCF-7 cell lysate, Jurkat cell lysate, A431 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat pancreas tissue lysate, human pancreas tissue, mouse pancreas tissue.

Molecular wt :

Predicted band size: 47 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC: 1:200

more info or order :

HUABIO product webpage