Phospho-eIF4G (S1108) | HUABIO HA750588 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-eIF4G (S1108)

catalog :

HA750588

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PS01-08

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-eIF4G (S1108) on different lysates with Rabbit anti-Phospho-eIF4G (S1108) antibody (HA750588) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 175 kDa Observed band size: 250 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750588) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue untreated / treated with λpp with Rabbit anti-Phospho-eIF4G (S1108) antibody (HA750588) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750588) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750588

Target name :

Phospho-eIF4G (S1108)

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide corresponding to Human eIF4G1 (phospho S1108).

Uniprot id :

Q04637>SwissProt: Q04637 Human

Host :

Rabbit

Clone number :

PS01-08

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, SK-Br-3 cell lysate, MCF-7 cell lysate, human ovary cancer tissue.

Molecular wt :

Predicted band size: 175 kDa

Subcellular location :

Cytosol. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage