product summary
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company name :
HUABIO
product type :
antibody
product name :
RbBP5
catalog :
HA750585
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH0-15
reactivity :
human, mouse, rat
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA750585 image 1
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA750585) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: C2C12 cell lysate Lane 4: 293 cell lysate Lane 5: MCF-7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: K562 cell lysate Lane 8: N2A cell lysate Lane 9: PC-12 cell lysate Lane 10: RAW264.7 cell lysate Lysates/proteins at 25 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750585) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA750585 image 2
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA750585) at 1/1,000 dilution. Lane 1: NIH-3T3-si NT cell lysate Lane 2: NIH-3T3-si RbBP5 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. HA721273 was shown to specifically react with RbBP5 in NIH-3T3-si NT cells. Weakened band was observed when NIH-3T3-si RbBP5 sample was tested. NIH-3T3-si NT and NIH-3T3-si RbBP5 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721273, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA750585
Target name :
RbBP5
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human RbBP5 aa 431-480 / 538.
Uniprot id :
Q15291>SwissProt: Q15291 Human;SwissProt: Q8BX09 Mouse;Entrez Gene: 304794 Rat
Host :
Rabbit
Clone number :
PSH0-15
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SW480 cell lysate, A431 cell lysate, C2C12 cell lysate, 293 cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, K562 cell lysate, N2A cell lysate, PC-12 cell lysate, RAW264.7 cell lysate.
Molecular wt :
Predicted band size: 59 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Knockdown (KD)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!