PSME2 | HUABIO HA750579 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PSME2

catalog :

HA750579

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH0-10

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PSME2 on different lysates with Rabbit anti-PSME2 antibody (HA750579) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si PSME2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750579) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PSME2 on different lysates with Rabbit anti-PSME2 antibody (HA750579) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (10 µg/Lane) Lane 2: 293T cell lysate (10 µg/Lane) Lane 3: Mouse liver tissue lysate (20 µg/Lane) Lane 4: Rat thymus tissue lysate (20 µg/Lane) Lane 5: MCF-7 cell lysate (10 µg/Lane) Lane 6: PC-3 cell lysate (10 µg/Lane) Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750579) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of Hela cells labeling PSME2 with Rabbit anti-PSME2 antibody (HA750579) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSME2 antibody (HA750579) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA750579

Target name :

PSME2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human aa 2-239.

Uniprot id :

Q9UL46>SwissProt: Q9UL46 Human;SwissProt: P97372 Mouse;SwissProt: Q63798 Rat

Host :

Rabbit

Clone number :

PSH0-10

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PC-12 cell lysate, 293T cell lysate, mouse liver tissue lysate, rat thymus tissue lysate, MCF-7 cell lysate, PC-3 cell lysate, Hela, rat stomach tissue, human rectal carcinoma tissue, HepG2.

Molecular wt :

Predicted band size: 27 kDa

Subcellular location :

Cytosol, Extracellular region or secreted, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:100 ;IHC-P: 1:4,000 ;FC: 1:500-1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750579_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-PSME2 antibody (HA750579) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750579) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750579_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human rectal carcinoma tissue with Rabbit anti-PSME2 antibody (HA750579) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750579) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750579_6.jpg

Pic legend6 :

Flow cytometric analysis of Hela cells labeling PSME2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750579, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750579_7.jpg

Pic legend7 :

Flow cytometric analysis of HepG2 cells labeling PSME2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750579, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage