CD15 | HUABIO HA750565 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD15

catalog :

HA750565

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PD00-42

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-CD15 antibody (HA750565) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750565) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 2 :

Flow cytometric analysis of human peripheral blood granulocytes labelling CD15 (HA750565).

image 3 :

Immunocytochemistry analysis of K-562 cells labeling CD15 with Rabbit anti-CD15 antibody (HA750565) at 1/20 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD15 antibody (HA750565) at 1/20 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750565

Target name :

CD15

Species reactivity :

Human

Applications :

IHC-P,FC,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Purified CD15 .

Uniprot id :

Host :

Rabbit

Clone number :

PD00-42

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human tonsil, Human breast carcinoma tissue, human peripheral blood granulocytes, K-562.

Molecular wt :

Predicted band size: 59 kDa

Subcellular location :

Golgi apparatus, Golgi stack membrane

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-P: 1:1,000 ;FC: 1:1,000 ;IF-Cell: 1:20

more info or order :

HUABIO product webpage