MALT1 | HUABIO HA750563 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MALT1

catalog :

HA750563

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-31

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (HA750563) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: Ramos cell lysate (15 µg/Lane) Lane 4: Daudi cell lysate (15 µg/Lane) Lane 5: K-562 cell lysate (15 µg/Lane) Lane 6: Mouse thymus tissue lysate (30 µg/Lane) Lane 7: Rat thymus tissue lysate (30 µg/Lane) Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 2 minutes 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750563) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MALT1 antibody (HA750563) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750563) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (HA750563) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: HepG2 cell lysate (20 µg/Lane) Lane 7: 786-0 cell lysate (20 µg/Lane) Lane 8: LNCaP cell lysate (20 µg/Lane) Lane 9: PC-3M cell lysate (20 µg/Lane) Lane 10: Mouse lymph node tissue lysate (no heat) (40 µg/Lane) Lane 11: Mouse thymus tissue lysate (40 µg/Lane) Lane 12: Rat thymus tissue lysate (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 37 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750563) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750563

Target name :

MALT1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human MALT1 aa 1-400 / 824 (Q9UDY8).

Uniprot id :

Q9UDY8>SwissProt: Q9UDY8 Human;SwissProt: Q2TBA3 Mouse;Entrez Gene: 307366 Rat

Host :

Rabbit

Clone number :

PSH02-31

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HCT 116 cell lysate, HeLa cell lysate, Ramos cell lysate, Daudi cell lysate, K-562 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate, human colon tissue, Jurkat cell lysate, HepG2 cell lysate, 786-0 cell lysate, LNCaP cell lysate, PC-3M cell lysate, Mouse lymph node tissue lysate, human colon cancer tissue.

Molecular wt :

Predicted band size: 92 kDa

Subcellular location :

Cytoplasm, perinuclear region, Nucleus

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750563_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MALT1 antibody (HA750563) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750563) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750563_5.jpg

Pic legend5 :

Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (HA750563) at 1/5,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si MALT1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 11 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750563) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage