KDM3B | HUABIO HA750508 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

KDM3B

catalog :

HA750508

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

1A5

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of KDM3B on different lysates with Rabbit anti-KDM3B antibody (HA750508) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 µg/Lane) Lane 2: NIH/3T3 cell lysate (25 µg/Lane) Predicted band size: 191 kDa Observed band size: 191 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750508) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of A431 cells labeling KDM3B with Rabbit anti-KDM3B antibody (HA750508) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (HA750508) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunocytochemistry analysis of Hela cells labeling KDM3B with Rabbit anti-KDM3B antibody (HA750508) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (HA750508) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA750508

Target name :

KDM3B

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human KDM3B aa 251-500.

Uniprot id :

Q7LBC6>SwissProt: Q7LBC6 Human;SwissProt: Q6ZPY7 Mouse

Host :

Rabbit

Clone number :

1A5

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela cell lysate, NIH/3T3 cell lysate, A431, Hela, human testis tissue, human fallopian tube tissue.

Molecular wt :

Predicted band size: 191 kDa

Subcellular location :

Nucleus

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000;IF-Cell: 1:100;IHC-P: 1:400-1:2,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750508_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3B antibody (HA750508) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750508) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750508_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-KDM3B antibody (HA750508) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750508) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage