ACSL4/FACL4 | HUABIO HA750469 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ACSL4/FACL4

catalog :

HA750469

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE56-33

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ACSL4/FACL4 on different lysates with Rabbit anti-ACSL4/FACL4 antibody (HA750469) at 1/5,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ACSL4/FACL4 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ACSL4/FACL4 on different lysates with Rabbit anti-ACSL4/FACL4 antibody (HA750469) at 1/1,000 dilution. Lane 1: PC-12 cell lysate Lane 2: C6 cell lysate Lane 3: L6 cell lysate Lane 4: Rat brain tissue lysate Lane 5: Rat lung tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of ACSL4/FACL4 on different lysates with Rabbit anti-ACSL4/FACL4 antibody (HA750469) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA750469

Target name :

ACSL4/FACL4

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human FACL4 aa 561-711 (Cytoplasmic).

Uniprot id :

O60488>SwissProt: O60488 Human;SwissProt: Q9QUJ7 Mouse;SwissProt: O35547 Rat

Host :

Rabbit

Clone number :

JE56-33

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PC-12 cell lysate, C6 cell lysate, L6 cell lysate, Rat brain tissue lysate, Rat lung tissue lysate, Rat thymus tissue lysate, A549 cell lysate, Mouse brain tissue lysate, mouse brain tissue, mouse lung tissue, rat brain tissue.

Molecular wt :

Predicted band size: 79 kDa

Subcellular location :

Peroxisome membrane, Mitochondrion outer membrane, Microsome membrane, Endoplasmic reticulum membrane, Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750469_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACSL4/FACL4 antibody (HA750469) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750469) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750469_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACSL4/FACL4 antibody (HA750469) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750469) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750469_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACSL4/FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750469, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage