Ctip1 | HUABIO HA750461 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ctip1

catalog :

HA750461

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB35-68

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Ctip1 on Jurkat cell lysates with Rabbit anti-Ctip1 antibody (HA750461) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 91 kDa Observed band size: 120 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750461) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Ctip1 on different lysates with Rabbit anti-Ctip1 antibody (HA750461) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 91 kDa Observed band size: 120 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750461) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of Jurkat cells labeling Ctip1 with Rabbit anti-Ctip1 antibody (HA750461) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ctip1 antibody (HA750461) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA750461

Target name :

Ctip1

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,FC,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Ctip1 aa 381-609 / 835.

Uniprot id :

Q9H165>SwissProt: Q9H165 Human;SwissProt: Q9QYE3 Mouse

Host :

Rabbit

Clone number :

JB35-68

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysates, Daudi cell lysate, Raji cell lysate, Jurkat, mouse brain tissue, mouse spleen tissue.

Molecular wt :

Predicted band size: 91 kDa

Subcellular location :

Cytoplasm. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:50 ;FC: 1:1,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750461_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Ctip1 antibody (HA750461) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750461) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750461_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Ctip1 antibody (HA750461) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750461) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750461_6.jpg

Pic legend6 :

Flow cytometric analysis of Jurkat cells labeling Ctip1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750461, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage