Iba1 | HUABIO HA750440 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Iba1

catalog :

HA750440

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM36-62

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/2,000 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Mouse Site: Hippocampus (APP-PS1 mouse) Sample: Frozen section Antibody concentration: 1/500 Date by conrtesy of: Mr. Chenxin Ma, School of Basic Medical Sicences, Zhejiang University

product information

SKU :

HA750440

Target name :

Iba1

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue,IF-Cell,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Iba1.

Uniprot id :

P55008>SwissProt: P55008 Human;SwissProt: O70200 Mouse;SwissProt: P55009 Rat

Host :

Rabbit

Clone number :

JM36-62

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, THP-1, J774A.1, RAW264.7, C6, mouse hippocampus tissue, human kidney tissue, human spleen tissue, mouse brain tissue, rat brain tissue.

Molecular wt :

Predicted band size: 17 kDa

Subcellular location :

Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:1,000 ;IHC-Fr: 1:1,000-1:2,000 ;IF-Tissue: 1:500 ;IF-Cell: 1:250-1:500 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750440_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/1,000 (Iba1, HA750440, Rabbit, red); 1/5,000 (c-Fos, HA601373, Rat, green) Antigen retrieval: Not required

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750440_5.jpg

Pic legend5 :

Application: IHC-Fr Species: Rat Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/1,000 (Iba1, HA750440, Rabbit, green); 1/1,000 (c-Fos, HA601373, Mouse, red) Antigen retrieval: Not required

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750440_6.jpg

Pic legend6 :

Application: IF-tissue Species: Human Site: Spleen Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA750440_7.jpg

Pic legend7 :

Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA750440_8.jpg

Pic legend8 :

Application: IF-tissue Species: Rat Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA750440_9.jpg

Pic legend9 :

Western blot analysis of Iba1 on different lysates with Rabbit anti-Iba1 antibody (HA750440) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750440) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA750440_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Iba1 antibody (HA750440) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA750440_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Iba1 antibody (HA750440) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA750440_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-Iba1 antibody (HA750440) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/HA750440_13.jpg

Pic legend13 :

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-Iba1 antibody (HA750440) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img14 :

https://storage.huabio.cn/huabio/productImg/HA750440_14.jpg

Pic legend14 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Iba1 antibody (HA750440) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img15 :

https://storage.huabio.cn/huabio/productImg/HA750440_15.jpg

Pic legend15 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Iba1 antibody (HA750440) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750440) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img16 :

https://storage.huabio.cn/huabio/productImg/HA750440_16.jpg

Pic legend16 :

Immunocytochemistry analysis of THP-1 cells labeling Iba1 with Rabbit anti-Iba1 antibody (HA750440) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (HA750440) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img17 :

https://storage.huabio.cn/huabio/productImg/HA750440_17.jpg

Pic legend17 :

Immunocytochemistry analysis of BV2 cells labeling Iba1 with Rabbit anti-Iba1 antibody (HA750440) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (HA750440) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img18 :

https://storage.huabio.cn/huabio/productImg/HA750440_18.jpg

Pic legend18 :

Immunocytochemistry analysis of RAW264.7 cells labeling Iba1 with Rabbit anti-Iba1 antibody (HA750440) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (HA750440) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img19 :

https://storage.huabio.cn/huabio/productImg/HA750440_19.jpg

Pic legend19 :

Immunocytochemistry analysis of C6 cells labeling Iba1 with Rabbit anti-Iba1 antibody (HA750440) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (HA750440) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img20 :

https://storage.huabio.cn/huabio/productImg/HA750440_20.jpg

Pic legend20 :

Flow cytometric analysis of THP-1 cells labeling Iba1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750440, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage