PDK1 | HUABIO HA750419 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PDK1

catalog :

HA750419

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JA67-30

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PDK1 on different lysates with Rabbit anti-PDK1 antibody (HA750419) at 1/5,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 49 kDa Observed band size: 46 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750419) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PDK1 on different lysates with Rabbit anti-PDK1 antibody (HA750419) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD PDK1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 49 kDa Observed band size: 46 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750419) at 1/1,000 dilution was used in primary antibody diluent at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-PDK1 antibody (HA750419) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750419) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750419

Target name :

PDK1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP,FC,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PDK1 aa 16-65 / 436.

Uniprot id :

Q15118>SwissProt: Q15118 Human;SwissProt: Q8BFP9 Mouse;SwissProt: Q63065 Rat

Host :

Rabbit

Clone number :

JA67-30

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse heart tissue lysate, rat heart tissue lysate, Hela, NIH/3T3, mouse heart tissue, rat heart tissue.

Molecular wt :

Predicted band size: 49 kDa

Subcellular location :

Mitochondrion matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:2,000 ;FC: 1:50-1:100 ;IF-Tissue: 1:200-1:400

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750419_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-PDK1 antibody (HA750419) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750419) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750419_5.jpg

Pic legend5 :

Flow cytometric analysis of PDK1 was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750419, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750419_6.jpg

Pic legend6 :

PDK1 was immunoprecipitated from 0.2 mg mouse heart tissue lysate with HA750419 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750419 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse heart tissue lysate (input) Lane 2: HA750419 IP in mouse heart tissue lysate Lane 3: Rabbit IgG instead of HA750419 in mouse heart tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801

more info or order :

HUABIO product webpage