CD163 | HUABIO HA750412 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD163

catalog :

HA750412

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JA51-30

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD163 on different lysates with Rabbit anti-CD163 antibody (HA750412) at 1/1,000 dilution. Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 125 kDa Observed band size: 150-170 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750412) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD163 antibody (HA750412) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750412) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD163 antibody (HA750412) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750412) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA750412

Target name :

CD163

Species reactivity :

Human

Applications :

WB,IHC-P,IF-Tissue,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human CD163 aa 1012-1149 / 1156.

Uniprot id :

Q86VB7>SwissProt: Q86VB7 Human

Host :

Rabbit

Clone number :

JA51-30

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human pancreatic carcinoma, human cervical cancer, human lung tissue lysates, human liver tissue lysates, human liver tissue, human spleen tissue, human placenta tissue, human tonsil tissue.

Molecular wt :

Predicted band size: 125 kDa

Subcellular location :

Cell membrane; Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:1,000-1:10,000 ;IF-Tissue: 1:200 ;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750412_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD163 antibody (HA750412) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750412) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA750412_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD163 antibody (HA750412) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750412) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA750412_6.jpg

Pic legend6 :

Application: IF-Tissue Species: Human Site: Spleen Sample: Paraffin-embedded section Antibody concentration: 1/200

more info or order :

HUABIO product webpage