FAP | HUABIO HA750409 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FAP

catalog :

HA750409

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JA56-11

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FAP on U-87 MG cell lysates with Rabbit anti-FAP antibody (HA750409) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 100 kDa Exposure time: 5 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750409) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-FAP antibody (HA750409) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750409) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunofluorescence analysis of frozen mouse colon tissue with Rabbit anti-FAP antibody (HA750409) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1704-23, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

product information

SKU :

HA750409

Target name :

FAP

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human FAP1 aa 1-160 / 760.

Uniprot id :

Q12884>SwissProt: Q12884 Human;SwissProt: P97321 Mouse;SwissProt: Q8R492 Rat

Host :

Rabbit

Clone number :

JA56-11

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

U-87 MG cell lysates, human pancreatic carcinoma, human colon cancer tissue.

Molecular wt :

Predicted band size: 88 kDa

Subcellular location :

Cell membrane. Cell surface.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000 ;IHC-Fr: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA750409_4.jpg

Pic legend4 :

Immunofluorescence analysis of frozen rat colon tissue with Rabbit anti-FAP antibody (HA750409) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1704-23, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage