FABP4 | HUABIO HA750398 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FABP4

catalog :

HA750398

quantity :

100μl

price :

649.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM10-99

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FABP4 on different lysates with Rabbit anti-FABP4 antibody (HA750398) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: Mouse white adipose tissue lysate Lane 2: Mouse brown adipose tissue lysate Lane 3: Mouse kidney tissue lysate (negative) Lane 4: Rat white adipose tissue lysate Lane 5: Rat brown adipose tissue lysate Lane 6: Rat kidney tissue lysate (negative) Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 3 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750398) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HAEC cells labeling FABP4 with Rabbit anti-FABP4 antibody (HA750398) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FABP4 antibody (HA750398) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunofluorescence analysis of frozen mouse adipose tissue labeling FABP4 with Rabbit anti-FABP4 antibody (HA750398). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (HA750398, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

product information

SKU :

HA750398

Target name :

FABP4

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human FABP4 aa 60-100.

Uniprot id :

P15090>SwissProt: P15090 Human;SwissProt: P04117 Mouse;SwissProt: P70623 Rat

Host :

Rabbit

Clone number :

JM10-99

Isotype :

IgG

Size :

100μl

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse white adipose tissue lysate, mouse brown adipose tissue lysate, rat white adipose tissue lysate, rat brown adipose tissue lysate, HAEC, MCF-7, A549, PMVEC.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus. Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100 ;IHC-Fr:1;100

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage