product summary
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company name :
HUABIO
product type :
antibody
product name :
CD26
catalog :
HA750396
quantity :
100μl
price :
649.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM11-42
reactivity :
human, mouse, rat
application :
western blot
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA750396 image 1
Western blot analysis of CD26 on different lysates with Rabbit anti-CD26 antibody (HA750396) at 1/5,000 dilution. Lane 1: LoVo cell lysate Lane 2: Caco-2 cell lysate Lane 3: Mouse thymus tissue lysate Lane 4: Rat thymus tissue lysate Lane 5: Mouse liver tissue lysate Lane 6: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 100/110 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750396) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA750396 image 2
All lanes: Western blot analysis of CD26 with anti-CD26 antibody (HA750396) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: CD26 knockout Hela whole cell lysate (10 µg). ET1703-93 was shown to specifically react with CD26 in wild-type Hela cells. NO band was observed when CD26 knockout sample was tested. Wild-type and CD26 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1703-93, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA750396
Target name :
CD26
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human CD26 aa 701-747 / 766 (Extracellular).
Uniprot id :
P27487>SwissProt: P27487 Human;SwissProt: P28843 Mouse;SwissProt: P14740 Rat
Host :
Rabbit
Clone number :
JM11-42
Isotype :
IgG
Size :
100μl
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
LoVo cell lysate, Caco-2 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver tissue, rat epididymis tissue.
Molecular wt :
Predicted band size: 88 kDa
Subcellular location :
Secreted, Cell membrane, Apical cell membrane, invadopodium membrane, lamellipodium membrane, Cell junction, Membrane raft.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000-1:5,000
Advanced Validation :
Knockout (KO)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!